A genomic library contains all the sequences present in the genome of an organism. In the construction of genomic libraries it is feasible to use vectors that could accommodate large size of inserts. The first step in the construction of genomic library is the isolation of genomic DNA, and entire DNA is subjected to restriction digestion. The fragmented DNA of [...]
In this article we will discuss about:- 1. Subject-Matter of Restriction Enzymes 2. Nomenclature of Restriction Enzymes 3. Types 4. Sites 5. Restriction Enzymes Generated Staggered and Blunt Ends 6. Purification. Subject-Matter of Restriction Enzymes: Restriction enzymes, also known as restriction endonucleases, have played a key role in the development of recombinant DNA technology. These have been found in microorganisms [...]
The following are the some of the examples of Somaclonal Variation: 1. Sugarcane 2. Potato 3. Tobacco 4. Rice 5. Barley. 1. Sugarcane: Application of somaclonal variation in crop improvement programme was established in sugarcane. The Hawaian Sugar Planters Association Experimental Station recorded genetic variation among sugarcane plants regenerated from tissue culture. Variations were recorded based on cytogenetic, morphological and [...]