In this article we will discuss about:- 1. Subject-Matter of Restriction Enzymes 2. Nomenclature of Restriction Enzymes 3. Types 4. Sites 5. Restriction Enzymes Generated Staggered and Blunt Ends 6. Purification. Subject-Matter of Restriction Enzymes: Restriction enzymes, also known as restriction endonucleases, have played a key role in the development of recombinant DNA technology. These have been found in microorganisms [...]
In this article we will discuss about:- 1. Meaning of Restriction Enzymes 2. Naming of Restriction Enzymes 3. Mechanism of Action. Meaning of Restriction Enzymes: These are also known as molecular scissors, used for cutting of DNA. The cutting of DNA at specific locations became possible with the discovery of molecular scissors, i.e., restriction enzymes. In the year 1963, the [...]
These are endonucleases which cleave double-stranded DNA molecules at specific sites having a sequence of generally four to six nucleotides. These sites are called restriction sites. A specific restriction enzyme cleaves DNA wherever the site occurs. The nucleotide sequence of the restriction site is specific for each of hundreds of restriction enzymes discovered so far from different microorganisms. A restriction [...]