In this article we will discuss about:- 1. Basic Strategies for Secondary Metabolite Production 2. Factors Affecting the Production of Secondary Metabolite Production 3. Specialized Strategies 4. Regulation. In the beginning of the plant tissue culture, studies were heavily focused on the fundamental assessment of nutrition, growth and differentiation. Several advancements in cell and tissue culture techniques have taken place [...]
The following points highlight the three types of nucleic acid probes. The probes are: 1. Oligonucleotide Probes 2. DNA Probes 3. RNA Probes. Type # 1. Oligonucleotide Probes: These are synthesized chemically as oligonucleotides based on the information available on the amino acid sequence of the protein of interest. These oligo nucleotides can be used as a probe the identification [...]
The sequence of a deoxyribonucleic acid DNA molecule can be elucidated using chemical or enzymatic methods. Chemical Method of DNA Sequencing: In 1997, Maxam and Gilbert of Harward University discovered this method. In this method, a DNA fragment to be sequenced is radiolabeled at one end of molecule (Fig. 13.15). After denaturation of DNA into single strand, bases are modified [...]