In this article we will discuss about:- 1. Basic Considerations of Nucleic Acid Hybridization 2. Hybridization Probes 3. Labeling of Hybridization Probes. Basic Considerations of Nucleic Acid Hybridization: Nucleic acid hybridization is a basic tool in molecular genetics which exploits the ability of single stranded nucleic acid molecules to hybridize with complementary sequences to form double stranded molecules. The principle [...]
Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) is a method to diagnose hereditary diseases caused by mutation not affecting restriction endonuclease sites. This method combines Polymerase Chain Reaction with the Ligation Assay. PCR-OLA distinguishes between the ligation and the absence of ligation of two oligonucleotides. A single nucleotide mismatch (at the site of hybridized oligonucleotides) prevents ligation and hence we can [...]
Application of Hardy-Weinberg law in calculating Gene (Allele) frequencies in a population. The gene frequencies for the autosomal and sex-chromosomal allele can be determined by the help of Hardy-Weinberg law by the following method: A. Calculation of Gene Frequencies of Autosomal Genes: An autosomal gene locus may have codominant alleles, dominant and recessive alleles or multiple alleles. If one desires [...]