In this article we will discuss about:- 1. Basic Considerations of Nucleic Acid Hybridization 2. Hybridization Probes 3. Labeling of Hybridization Probes. Basic Considerations of Nucleic Acid Hybridization: Nucleic acid hybridization is a basic tool in molecular genetics which exploits the ability of single stranded nucleic acid molecules to hybridize with complementary sequences to form double stranded molecules. The principle [...]
Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) is a method to diagnose hereditary diseases caused by mutation not affecting restriction endonuclease sites. This method combines Polymerase Chain Reaction with the Ligation Assay. PCR-OLA distinguishes between the ligation and the absence of ligation of two oligonucleotides. A single nucleotide mismatch (at the site of hybridized oligonucleotides) prevents ligation and hence we can [...]
Regarding the genetic structure of the population, the following two hypotheses have been proposed:- 1. Classical Hypothesis 2. Balance Hypothesis. Model # 1. Classical Hypothesis: It was developed by T.H. Morgan (1932) and supported by H.J. Muller and Kaplan (1966). The classical hypothesis proposes that the gene pool of a population consists at each gene locus of a wild-type allele [...]