The following are the some of the examples of Somaclonal Variation: 1. Sugarcane 2. Potato 3. Tobacco 4. Rice 5. Barley. 1. Sugarcane: Application of somaclonal variation in crop improvement programme was established in sugarcane. The Hawaian Sugar Planters Association Experimental Station recorded genetic variation among sugarcane plants regenerated from tissue culture. Variations were recorded based on cytogenetic, morphological and [...]
In this article we will discuss about:- 1. Discovery and Definition of Somaclonal Variation 2. Techniques to Generate Somaclonal Variations 3. Selection and Isolation 4. Screening 5. Applications. Discovery and Definition of Somaclonal Variation: Improvement of plants through alterations or additions of traits are some of the main objectives in plant biotechnology. Plant breeders have recognized the potential sources of [...]
The following points highlight the five main steps for aseptic manipulation. The steps are: 1. Sterilisation of the Culture Vessels and Instruments 2. Sterilisation of Nutrient Media 3. Sterilisation of Culture Rooms and Transfer-Area 4. Sterilisation of Explant 5. Aseptic Transfer of Explant/Sub-Culturing. Step # 1. Sterilisation of the Culture Vessels and Instruments: For sterilisation of culture vessels, glassware's, instruments, [...]