In this article we will discuss about the Tissue Culture:- 1. Definition of Tissue Culture 2. History of Tissue Culture 3. Importance. Definition of Tissue Culture: Tissue culture is the method of 'in vitro' culture of plant or animal cells, tissue or organ - on nutrient medium under aseptic conditions usually in a glass container. Tissue culture is sometimes referred [...]
The following points highlight the four main aspects required for plant tissue culture. The aspects are: 1. Aseptic Condition 2. Aeration 3. Equipment 4. Nutrient Medium. Aspect # 1. Aseptic Condition: Tissue culture should be done in completely aseptic condition. Care should be taken so that the tissues, equipment, culture media and the room should be completely free from microorganisms. [...]
The following points highlight the six important methods of tissue culture. The methods are: 1. Hanging Drop Culture 2. Double Cover Slip Method 3. Culture in Stewards Auxophyton 4. Culture in Bottles, Conical Flasks etc. 5. Culture in a Micro-Chamber 6. Culture in Petridish, Watch Glass etc.. Method # 1. Hanging Drop Culture: The simplest method of tissue culture is [...]