Tag Archives | Plant Biotechnology

Transformation Process of Plants | Genetics

Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Transformation Process of Plants | Genetics
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Polymerase Chain Reaction (PCR) | Genetics

In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This direct enzymatic amplification process is [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Polymerase Chain Reaction|Comments Off on Polymerase Chain Reaction (PCR) | Genetics
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Role of RNA in Transcription Regulation | Nucleic Acids

In this article we will discuss about the role of RNA in transcription regulation. In the early 1960s, Francois Jocob and Jaques Monod performed exemplary work per­taining to prokaryotic gene regulation. These experimental techniques and strategies were later extended to eukaryotic system. Although control of transcription between prokaryotic and eukaryotic genes shares substantial homology, eukaryotic control of system is highly [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Nucleic Acid|Comments Off on Role of RNA in Transcription Regulation | Nucleic Acids
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