Tag Archives | Plant Biotechnology

Methods to Create Marker Gene-Free Transformed Plants | Genetics

Four main methods have been suggested to create marker gene-free transformed plants. The methods are: 1. Co-Transformation 2. Site-Specific Recombination 3. Marker Gene Elimination by Transposase 4. Marker Gene Elimination by Intrachromosomal Recombination. Method # 1. Co-Transformation: Co-transformation strategies are popularly employed for the production of transformed plants free from marker gene(s). In this novel approach, gene of interest and [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Methods to Create Marker Gene-Free Transformed Plants | Genetics
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Agrobacterium as Gene Transformation Vector | Genetics

One of the significant developments in agriculture biotechnology is the successful transformation of plants by Agrobacterium as a vector. This has formed a basis for the better performance of plants. Existence of close proximity between plant and Agrobacterium leads to the exploitation of these bacteria for the production of transgenic plants. In addition, accumulation of substantial amount of information regarding [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Agrobacterium as Gene Transformation Vector | Genetics
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Precision of Transgene Integration | Genetics

Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control over the impact of genomic [...]

By |2016-12-23T06:35:55+00:00December 23, 2016|Genetics|Comments Off on Precision of Transgene Integration | Genetics
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