Tag Archives | Plant Biotechnology

Precision of Transgene Integration | Genetics

Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control over the impact of genomic [...]

By |2016-12-23T06:35:55+00:00December 23, 2016|Genetics|Comments Off on Precision of Transgene Integration | Genetics

Transformation Process of Plants | Genetics

Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Transformation Process of Plants | Genetics

Polymerase Chain Reaction (PCR) | Genetics

In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This direct enzymatic amplification process is [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Polymerase Chain Reaction|Comments Off on Polymerase Chain Reaction (PCR) | Genetics
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