Gene transfer in cereals is becoming established at the backdrop of recombinant DNA technology. Until recently, gene transfer in plants was accomplished only through certain conventional methods like sexual hybridization. The entire scenario of transformation was changed after the advancement of genetic engineering. Utility of Agrobacterium tumeifaciens as biological vector was found to be successful among dicots. However, its potential [...]
The following points highlight the two types of gel electrophoresis. The types are: 1. Polyacrylamide Gel Electrophoresis 2. Agarose Gel Electrophoresis Gel electrophoresis is the novel technique in which nucleic acid (even proteins) molecules are separated based on the size differences when subjected to electric field. In electrophoresis, the rate of migration in electric field depends on the field, net [...]
In this article we will discuss about:- 1. Basic Strategies for Secondary Metabolite Production 2. Factors Affecting the Production of Secondary Metabolite Production 3. Specialized Strategies 4. Regulation. In the beginning of the plant tissue culture, studies were heavily focused on the fundamental assessment of nutrition, growth and differentiation. Several advancements in cell and tissue culture techniques have taken place [...]