The following points highlight the two types of gel electrophoresis. The types are: 1. Polyacrylamide Gel Electrophoresis 2. Agarose Gel Electrophoresis Gel electrophoresis is the novel technique in which nucleic acid (even proteins) molecules are separated based on the size differences when subjected to electric field. In electrophoresis, the rate of migration in electric field depends on the field, net [...]
In this article we will discuss about the role of RNA in transcription regulation. In the early 1960s, Francois Jocob and Jaques Monod performed exemplary work pertaining to prokaryotic gene regulation. These experimental techniques and strategies were later extended to eukaryotic system. Although control of transcription between prokaryotic and eukaryotic genes shares substantial homology, eukaryotic control of system is highly [...]
In this article we will discuss about the process of in situ hybridization in nucleic acids. The mammalian and other higher eukaryons system contains larger DNA molecule. Some times larger than 50,000 kb. Hence, non-conventional methods like gel electrophoresis is not feasible. The gene location on these larger molecules can be identified by in situ hybridization which can prove accurate [...]