One of the significant developments in agriculture biotechnology is the successful transformation of plants by Agrobacterium as a vector. This has formed a basis for the better performance of plants. Existence of close proximity between plant and Agrobacterium leads to the exploitation of these bacteria for the production of transgenic plants. In addition, accumulation of substantial amount of information regarding [...]
Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control over the impact of genomic [...]
Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]