Tag Archives | Plant Biotechnology

Factors Affecting Micro-Propagation | Plants

The following points highlight the three main factors affecting micro-propagation. The factors are: 1. Genotype 2. Media 3. Cultural Conditions. Factor # 1. Genotype: Physiological conditions of the donor plants influence considerably in the process of morphogenesis. Larger sizes of the meristematic tissues are advantageous. Selecting juvenile parts of the plants yield better results than older ones. The most actively [...]

By |2016-12-23T06:34:57+00:00December 23, 2016|Micro-Propagation|Comments Off on Factors Affecting Micro-Propagation | Plants

Blotting Techniques Used for Gene Expression | Genetics

Blotting technique is an extremely powerful tool for analyzing gene structure and used to study gene expression, once cloned cDNA is isolated. There are three important types of blotting techniques are: 1. Southern Blotting 2. Northern Blotting 3. Western Blotting. Technique # 1. Southern Blotting: Developed by E.M. Southern, the technique of Southern blotting is one of the most imĀ­portant [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Gene Expression|Comments Off on Blotting Techniques Used for Gene Expression | Genetics

Methods to Create Marker Gene-Free Transformed Plants | Genetics

Four main methods have been suggested to create marker gene-free transformed plants. The methods are: 1. Co-Transformation 2. Site-Specific Recombination 3. Marker Gene Elimination by Transposase 4. Marker Gene Elimination by Intrachromosomal Recombination. Method # 1. Co-Transformation: Co-transformation strategies are popularly employed for the production of transformed plants free from marker gene(s). In this novel approach, gene of interest and [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off on Methods to Create Marker Gene-Free Transformed Plants | Genetics
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