Generally, isolation of protoplast is categorized into mechanical and enzymatic. Also learn about the techniques involved in the isolation of leaf mesophyll protoplast. Mechanical Method: This method is crude and oldest method for protoplast isolation. It is only an historical perspective. It involves chopping and plasmolysis of tissues. Time consuming and low yield of protoplast are the main constraints in [...]
One of the significant developments in agriculture biotechnology is the successful transformation of plants by Agrobacterium as a vector. This has formed a basis for the better performance of plants. Existence of close proximity between plant and Agrobacterium leads to the exploitation of these bacteria for the production of transgenic plants. In addition, accumulation of substantial amount of information regarding [...]
Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control over the impact of genomic [...]