The following points highlight the six main screening strategies that are to be enforced for the selection of ideal somatic hybrids. The strategies are: 1. Microscopic Visual Selection 2. Auxin Autonomy 3. Chlorophyll Complementation 4. Biochemical Selection 5. Verification by Molecular Screening 6. Chromosomal Analysis. Strategy # 1. Microscopic Visual Selection: It is based on the fusion between coloured and [...]
In this article we will discuss about:- 1. Subject Matter of CamV35S Promoter 2. Molecular Dissection of CamV35S Promoter 3. Functional Regions. Subject Matter of CamV35S Promoter: The 35S promoter is one of the major promoters of cauliflower mosaic virus (CamV). This virus infects crucifers and contains double-stranded genome as the genetic material. Based on the 8 kb genetic map, [...]
Developments of promoters that are activated in response to a specific chemical are particularly desirable in plant transformation. Utility of chemically inducible promoters are increasingly relevant to the improvement of crops by genetic engineering. Besides, several biotechnological applications of chemically inducible promoters have been witnessed recently such as construction of a male sterile system, expression of transgene that interfere with [...]