Archive | DNA Sequencing

Methods of DNA Sequencing | Nucleic Acids

The sequence of a deoxyribonucleic acid DNA molecule can be elucidated using chemical or enzymatic methods. Chemical Method of DNA Sequencing: In 1997, Maxam and Gilbert of Harward University discovered this method. In this method, a DNA fragment to be sequenced is radiolabeled at one end of molecule (Fig. 13.15). After denaturation of DNA into single strand, bases are modified [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|DNA Sequencing|Comments Off on Methods of DNA Sequencing | Nucleic Acids
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DNA Sequencing: 7 Methods Used for DNA Sequencing

This article throws light upon the seven important methods used for DNA sequencing. The seven important methods used for DNA sequencing are: (1) Sanger's Method (2) Maxam and Gilbert Method (3) Hybridization Method (4) Pal Nyren's Method (5) Automatic DNA Sequencer (6) Slab Gel Sequencing Systems and (7) Capillary Gel Electrophoresis. DNA sequencing is the determination of the precise sequence [...]

By |2015-10-16T06:35:48+00:00October 1, 2015|DNA Sequencing|Comments Off on DNA Sequencing: 7 Methods Used for DNA Sequencing
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