Archive | Plant Biotechnology

Rapid Gene Regulation by Auxin | Biotechnology

In this article we will discuss about the rapid gene regulation by auxin. Naturally occurring hormone indole acetic acid (IAA) is the fastest known hormonal action among plant in terms of rapid cell elongation within a short span of 10-20 minutes. Auxin exhibits two types of response. One is short-term effect with 20 min leads to cell elonga­tion. Second is [...]

By |2016-12-23T06:34:58+00:00December 23, 2016|Plant Biotechnology|Comments Off on Rapid Gene Regulation by Auxin | Biotechnology

Preparation of Culture Media | Biotechnology

In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]

By |2016-12-23T06:34:57+00:00December 23, 2016|Plant Biotechnology|Comments Off on Preparation of Culture Media | Biotechnology

Techniques of Protoplast Culture | Biotechnology

The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]

By |2016-12-23T06:34:57+00:00December 23, 2016|Protoplast Culture|Comments Off on Techniques of Protoplast Culture | Biotechnology
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