In this article we will discuss about the rapid gene regulation by auxin. Naturally occurring hormone indole acetic acid (IAA) is the fastest known hormonal action among plant in terms of rapid cell elongation within a short span of 10-20 minutes. Auxin exhibits two types of response. One is short-term effect with 20 min leads to cell elongation. Second is [...]
According to Murashige of California University, micro-propagation is distinctly divided into five stages. Stage 0 — Mother Plant Selection: Before initiation of micro-propagation, selection of suitable mother plant is crucial in the whole exercise of propagation. Generally, disease free mother explant is selected for the micro-propagation to reduce contamination of cultures. Certain growth parameters of mother plant can be improved [...]
In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]