In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]
The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]
In this article we will discuss about the significance of protoplast culture. Cell Modification by DNA Uptake: The isolated plant protoplasts are best suited for the introduction of foreign genetic material. Protoplast acts as an efficient receptor for the uptake of foreign DNA. Protoplast can be regenerated into the whole plant and this is indispensable for genetic manipulation. The ratio [...]