In this article we will discuss about:- 1. Anther Culture Technique 2. Microspore Culture 3. Diploitization 4. Anther Vs. Pollen Culture 5. Plant Regeneration 6. Characteristics of Microspore Derived Plants 7. Ontogeny of Androgenesis. The term haploid refers to those plants which contains gametophytic number of chromosomes. Anther/pollen culture mediated production of haploid plants approves the concept of totipotency. Guha [...]
Plant culture media contains: 1. Macronutrients 2. Micronutrients 3. Adjuvant 4. Carbon Source. 1. Macronutrients: Macronutrients or macroelements are basically inorganic salts. There are six major elements indispensable for growth of plants—nitrogen, phosphorus, potassium, calcium, magnesium and sulphur. Carbon, hydrogen and oxygen are obtained from the environment. In culture media concentration of these elements are well balanced depending on the [...]
According to Murashige of California University, micro-propagation is distinctly divided into five stages. Stage 0 — Mother Plant Selection: Before initiation of micro-propagation, selection of suitable mother plant is crucial in the whole exercise of propagation. Generally, disease free mother explant is selected for the micro-propagation to reduce contamination of cultures. Certain growth parameters of mother plant can be improved [...]