Archive | Plant Biotechnology

Composition of Plant Culture Media | Biotechnology

Plant culture media contains: 1. Macronutrients 2. Micronutrients 3. Adjuvant 4. Carbon Source. 1. Macronutrients: Macronutrients or macroelements are basically inorganic salts. There are six major ele­ments indispensable for growth of plants—nitrogen, phosphorus, potassium, calcium, magne­sium and sulphur. Carbon, hydrogen and oxygen are obtained from the environment. In culture media concentration of these elements are well balanced depending on the [...]

By |2016-12-23T06:34:57+00:00December 23, 2016|Plant Biotechnology|Comments Off on Composition of Plant Culture Media | Biotechnology
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Stages of Micro-Propagation | Plants

According to Murashige of California University, micro-propagation is distinctly divided into five stages.  Stage 0 — Mother Plant Selection: Before initiation of micro-propagation, selection of suitable mother plant is crucial in the whole exercise of propagation. Generally, disease free mother explant is selected for the micro-propagation to reduce contamination of cultures. Certain growth parameters of mother plant can be improved [...]

By |2016-12-23T06:34:57+00:00December 23, 2016|Plant Biotechnology|Comments Off on Stages of Micro-Propagation | Plants
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Preparation of Culture Media | Biotechnology

In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]

By |2016-12-23T06:34:57+00:00December 23, 2016|Plant Biotechnology|Comments Off on Preparation of Culture Media | Biotechnology
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