However, even after transformation, it is essential to confirm the integration of genes, for the success of transgenesis. The incorporation can be confirmed through (i) The use of markers, (ii) Southern blotting, (iii) Northern blotting, and (iv) Polymerase chain reaction. (i) Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. It is, therefore, important that [...]
The following points highlight the top five benefits of transgenic animals. The benefits are: 1. Normal Physiology and Development 2. Study of Disease 3. Biological Products 4. Vaccine Safety 5. Chemical Safety Testing. Benefit # 1. Normal Physiology and Development: Transgenic animals can be specifically designed to allow the study of how genes are regulated and how they affect the [...]
The following points highlight the seven main stages of recombinant DNA technology. The stages are: 1. Isolation of the Genetic Material (DNA) 2. Cutting of DNA at Specific Locations 3. Isolation of Desired DNA Fragment 4. Amplification of Gene of Interest using PCR 5. Ligation of DNA Fragment into a Vector 6. Insertion of Recombinant DNA into the Host Cell/Organisms [...]