The following points highlight the four main features that are required to facilitate cloning into a vector. The features are: 1. Origin of Replication 2. Selectable Marker 3. Cloning (Recognition) Sites 4. Vectors for Cloning Genes in Plants and Animals. Feature # 1. Origin of Replication (Ori): This is a sequence from where replication starts and any piece of DNA, [...]
In this article we will discuss about:- 1. Meaning of Restriction Enzymes 2. Naming of Restriction Enzymes 3. Mechanism of Action. Meaning of Restriction Enzymes: These are also known as molecular scissors, used for cutting of DNA. The cutting of DNA at specific locations became possible with the discovery of molecular scissors, i.e., restriction enzymes. In the year 1963, the [...]
Are you researching experiments on biotechnology ? You are in the right place. The below mentioned article includes a collection of seven experiments on biotechnology: 1. Culture Media 2. Callus Tissue Culture 3. Tissue Culture Medium 4. Tissue Culture in Plants 5. Isolation of Single Cells 6. Cell Planting Technique 7. Isolation of Protoplasm. Contents: Experiment on Culture Media Experiment [...]