Methods of introducing foreign genes in cell are: 1. Electroporation 2. Particle Bombardment Gun 3. Microinjection. Method # 1. Electroporation: In this method an electric field is used to transfer the foreign DNA into the fragile cells. High voltage electricity is applied to the protoplast suspension containing recombinant or naked plasmids. The electrical impulse creates large pores in the cell [...]
Genetic modification of rumen bacteria by using rDNA technology may improve the rate or extent of lignocellulolysis in the animal. The task of developing effective rDNA technology in rumen bacteria, particularly for improving lignocellulose digestion is complex. After the rate limiting steps have been defined, it is necessary to identify and clone suitable heterologous genes for insertion into a rumen [...]
In this article we will discuss about the applications of PCR. i. Detection of mutations: PCR is useful for detection of mutations related to genetic disease including point mutations, insertions and deletions. A notable example is that of the human dystrophin gene associated with Duchenne muscular dystrophy. The large-sized dystrophin gene consists of about two million base pairs located on [...]