Methods of introducing foreign genes in cell are: 1. Electroporation 2. Particle Bombardment Gun 3. Microinjection. Method # 1. Electroporation: In this method an electric field is used to transfer the foreign DNA into the fragile cells. High voltage electricity is applied to the protoplast suspension containing recombinant or naked plasmids. The electrical impulse creates large pores in the cell [...]
Genetic modification of rumen bacteria by using rDNA technology may improve the rate or extent of lignocellulolysis in the animal. The task of developing effective rDNA technology in rumen bacteria, particularly for improving lignocellulose digestion is complex. After the rate limiting steps have been defined, it is necessary to identify and clone suitable heterologous genes for insertion into a rumen [...]
Introduction to Recombinant DNA Technology: Recombinant DNA technology has revolutionised life sciences, opening new vistas for research in molecular biology. It allows genetic manipulation using techniques for synthesizing, amplifying and purifying individual genes from any type of cell. Genomics has emerged as an extension of recombinant DNA technology for high resolution mapping and characterization of whole genomes and gene products [...]