In this article we will discuss about the vectors of gene transfer. The common features of vectors used for transformation of eukaryotic cells include: (i) Multiple cloning sites (unique restriction site), (ii) Bacterial origin of replication, and (iii) Selectable marker gene which will allow to select the proper transformed cell. Ti and Ri Plasmid: The potential use of the Agrobacterium [...]
The following points highlight the top two methods of gene transfer. The methods are: 1. Indirect Method (Agrobacterium Mediated Gene Transfer) 2. Direct Methods of Gene Transfer. Method # 1. Indirect Method (Agrobacterium Mediated Gene Transfer): The transformation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in [...]
The below mentioned article provides a study note on transgenic plants. The application of recombinant technique for horizontal transfer of gene in plant sysÂtem has several limitations. Plant genome is exceedingly complex, involving a huge amount of DNA, which can accommodate sequences equivalent to 6-7 million genes in an average crop plant. Several genes are located outside the nucleus, namely [...]