The following points highlight the six significant techniques of gene transfer by help of agrobacterium tumefaciens. The techniques are: 1. Regeneration of Plants from Protoplasts and Callus Tissues 2. The Ti plasmid of Agrobacterium Tumefaciens 3. Formation of Non-oncogenic (or Disarmed) Ti Plasmids 4. Chimeric Selectable Marker Genes and a few others. Technique # 1. Regeneration of Plants from Protoplasts [...]
The following points highlight the top four types of direct gene transfer in plants. The types are: 1. Plant Cell Transformation by Ultra-Sonication 2. Liposome Mediated Gene Transfer 3. Electroporation 4. Particle Bombardment Gun. Type # 1. Plant Cell Transformation by Ultra-Sonication: The Biotechnology Research Centre, Beijing (China) has used technique of ultra-sonication for gene transfer in plants such as [...]
The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound Mediated Transfer. Method # 1. Biolystic or Particle Bombardment: [...]