The following points highlight the six significant techniques of gene transfer by help of agrobacterium tumefaciens. The techniques are: 1. Regeneration of Plants from Protoplasts and Callus Tissues 2. The Ti plasmid of Agrobacterium Tumefaciens 3. Formation of Non-oncogenic (or Disarmed) Ti Plasmids 4. Chimeric Selectable Marker Genes and a few others. Technique # 1. Regeneration of Plants from Protoplasts [...]
The following points highlight the top four types of direct gene transfer in plants. The types are: 1. Plant Cell Transformation by Ultra-Sonication 2. Liposome Mediated Gene Transfer 3. Electroporation 4. Particle Bombardment Gun. Type # 1. Plant Cell Transformation by Ultra-Sonication: The Biotechnology Research Centre, Beijing (China) has used technique of ultra-sonication for gene transfer in plants such as [...]
The structure of proteins can be altered by three types of mutations: 1. Point Mutations: Point mutations involve addition, deletion or replacement of base pair in a gene. 2. Chromosomal Mutations: Addition or deletion of DNA cause mutations during crossing over. 3. Jumping genes also cause change in protein structure by shuffling their location from one chromosome to another. The [...]