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Gene Expression in Plants | Biotechnology

In this article we will discuss about:- 1. Gene Expression in Vegetative Organs 2. Gene Expression in Reproductive Organs 3. Gene Expression in Seeds 4. Gene Expression in Fruits. Successful studies on gene expression in plants are undoubtedly due to the contribution of early botanical monograph in which cytological structure and function of organs were deĀ­fined. With the advent of [...]

By |2016-12-23T06:34:58+00:00December 23, 2016|Gene Expression|Comments Off on Gene Expression in Plants | Biotechnology

Blotting Techniques Used for Gene Expression | Genetics

Blotting technique is an extremely powerful tool for analyzing gene structure and used to study gene expression, once cloned cDNA is isolated. There are three important types of blotting techniques are: 1. Southern Blotting 2. Northern Blotting 3. Western Blotting. Technique # 1. Southern Blotting: Developed by E.M. Southern, the technique of Southern blotting is one of the most imĀ­portant [...]

By |2016-12-23T06:34:56+00:00December 23, 2016|Gene Expression|Comments Off on Blotting Techniques Used for Gene Expression | Genetics

Physical Methods of Gene Transfer | Genetics

The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound Mediated Transfer. Method # 1. Biolystic or Particle Bombardment: [...]

By |2016-12-23T06:34:55+00:00December 23, 2016|Gene Transfer|Comments Off on Physical Methods of Gene Transfer | Genetics
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