The below mentioned article provides notes on overlapping gene. In 1940s, Beadle and Tatum proposed one-gene-one protein hypothesis which explains that one gene encodes for one protein. However, if one gene consists of 1,500 base pairs, a protein of 500 amino acids in length would be synthesized. In addition, if the same sequence read in two different ways, two different [...]
In this article we will discuss about the discovery and evolution of split genes. Discovery of Split Genes: During 1970, in some mammalian viruses (e.g. adenoviruses) it was found that the DNA sequences coding for a polypeptide were not present continuously but were split into several pieces. Therefore, these genes were variously named as split genes or introns, interrupted genes [...]
Some procedures applied for gene cloning in eukaryotes are described in this context: 1. Transformation of Filamentous Fungi 2. Gene Transfer through Ti-plasmid of Agrobacterium 3. Gene Transfer through Gene Gun 4. Gene Bank or Genomic Library. 1. Transformation of Filamentous Fungi: Like bacteria, transformation of filamentous fungi has also been demonstrated. Hinnen (1978) first described transformation of protoplasts of [...]