Let us make an in-depth study of the gene therapy strategies designed to interfere with the HIV-1 life-cycle. Basically Inhibition has been Envisaged at Three Major Levels: (i) Blocking HIV-1 infection: HIV-1 normalÂly infects T lymphocytes by binding of the viral gp120 envelope protein to the CD4 receptor on the cell membrane. Transfer of a gene encoding a soluble form [...]
In prokaryotes several mechanisms viz., transformation, conjugation and transduction have been described that mediate the formation of new recombinants. Transformation refers to transfer of relatively small segment of naked DNA from a donor cell (male) to the recipient (female) cell. Conjugation is the process of gene transfer between cells of opposite mating types that are in physical contact with each [...]
The below mentioned article provides notes on overlapping gene. In 1940s, Beadle and Tatum proposed one-gene-one protein hypothesis which explains that one gene encodes for one protein. However, if one gene consists of 1,500 base pairs, a protein of 500 amino acids in length would be synthesized. In addition, if the same sequence read in two different ways, two different [...]