A genome library consists of a large collection of bacterial cultures, in each of which a different segment of the genomic DNA of an organism has been cloned by recombinant DNA technology, so that the entire collection represents its complete genome. The analogy between a library of books and a genome library is based on the fact that both genes [...]
In this article we will discuss about the three main types of vectors used in cloning for larger molecules of DNA. The types of vectors are: 1. Plasmid Vectors 2. Bacteriophage Vectors 3. Cosmid Vectors. 1. Plasmid Vectors: Plasmids are the extra chromosomal genetic elements commonly found in bacteria and are mostly made of double-stranded circular DNA. They are used [...]
In this article we will discuss about the top two techniques used for introduction of foreign DNA without vectors. The techniques are: 1. Electroporation Technique 2. Microinjection Technique Some direct methods have also been developed for transferring DNA of one organism to a recipient without the participation of a vector. Two such methods are: 1. Electroporation technique and 2. Microinjection [...]