In this article we will discuss about the steps and applications of Recombinant DNA Technology. Steps Involved in Recombinant DNA Technology: The sequences of steps in r-DNA technology are as follows: (i) Cell culture of cells with the required DNA sequences is obtained through tissue culture technique, (ii) Specific restriction endonuclease is selected from the specific bacterium, (iii) Restriction endonuclease [...]
The following points highlight the three modes of gene transfer and genetic recombination in bacteria. The modes are: 1. Transformation 2. Transduction 3. Bacterial Conjugation. Mode # 1. Transformation: Historically, the discovery of transformation in bacteria preceded the other two modes of gene transfer. The experiments conducted by Frederick Griffith in 1928 indicated for the first time that a gene-controlled [...]
In this article we will discuss about the top five techniques used for cloning of specific genes and their identification. The techniques are: 1. Direct Selection of Recombinant Clones 2. Colony Hybridization 3. Complementary DNA (c-DNA) 4. Isolation of a Specific Gene: Southern Blotting 5. Polymerase Chain Reaction (PCR). The restriction digest always contains an assortment of donor DNA fragments [...]