The following points highlight the seven main stages of recombinant DNA technology. The stages are: 1. Isolation of the Genetic Material (DNA) 2. Cutting of DNA at Specific Locations 3. Isolation of Desired DNA Fragment 4. Amplification of Gene of Interest using PCR 5. Ligation of DNA Fragment into a Vector 6. Insertion of Recombinant DNA into the Host Cell/Organisms [...]
The following points highlight the four main features that are required to facilitate cloning into a vector. The features are: 1. Origin of Replication 2. Selectable Marker 3. Cloning (Recognition) Sites 4. Vectors for Cloning Genes in Plants and Animals. Feature # 1. Origin of Replication (Ori): This is a sequence from where replication starts and any piece of DNA, [...]
In this article we will discuss about:- 1. Meaning of Restriction Enzymes 2. Naming of Restriction Enzymes 3. Mechanism of Action. Meaning of Restriction Enzymes: These are also known as molecular scissors, used for cutting of DNA. The cutting of DNA at specific locations became possible with the discovery of molecular scissors, i.e., restriction enzymes. In the year 1963, the [...]