Recombinant DNA has opened new avenues for the study of gene function. The function of a cloned gene can be investigated by introducing the cloned DNA into eukaryotic cells. In simple eukaryotes such as yeasts Saccharomyces cerevisiae, molecular clones of various mutants have been prepared for genetic analysis. Like bacteria, yeasts offer advantages in having a small genome of about [...]
In this article we will discuss about the meaning an classes of DNA polymorphisms. Meaning of DNA Polymorphisms: Different alleles of a gene produce different phenotypes which can be detected by making crosses between parents with different alleles of two or more genes. Then by determining recombinants in the progeny, a genetic map can be deduced. These are low resolution [...]
In this article we will discuss about:- 1. Definition of Recombinant DNA 2. History of Recombinant DNA 3. Milestones. Definition of Recombinant DNA: For centuries humans have been altering the genetic makeup of organisms by selective breeding of plant and animals. The deliberate modification in genetic material of an organism by changing the nucleic acid directly is called genetic engineering [...]