In this article we will discuss about the role of Ti plasmids in genetic engineering of plants. Genetic engineering of plants is carried out by introducing DNA into a cell in culture that can grow into a mature plant. An efficient vector for introduction of recombinant DNA into plant cells has been developed from plant viruses. Furthermore, the Ti plasmid [...]
In this article we will discuss about the determination of DNA sequence. The sequence of bases A, T, G, and C in genes and whole genome can provide useful information for understanding gene structure and organisation, encoded protein and cell behaviour. The first complete nucleotide sequence of an entire viral genome (φX174) was determined in 1977 in the laboratory of [...]
Introduction to Recombinant DNA Technology: Recombinant DNA technology has revolutionised life sciences, opening new vistas for research in molecular biology. It allows genetic manipulation using techniques for synthesizing, amplifying and purifying individual genes from any type of cell. Genomics has emerged as an extension of recombinant DNA technology for high resolution mapping and characterization of whole genomes and gene products [...]