In this article we will discuss about the meaning of transgenic mice. Cloned genes can be introduced into the germ line cells of an animal allowing study of the intact animal rather than in cultured cells. For this purpose, the nucleus in mouse embryonal cells has been used for injecting foreign DNA. The goal in such experiments is to have [...]
In this article we will discuss about the uses of DNA microarrays. The DNA Microarray technology is used to determine the level of expression of many thousands of genes simultaneously. This new approach is used not for individual genetic loci, rather, for the analysis of genome-wide patterns of gene expression. Using DNA microarrays, it is possible to estimate the relative [...]
Cloned genes can be introduced into knockout mice by means of embryonal stem cells (ES cells). Embryonal cells have attracted tremendous attention in recent years, hence a brief introduction on ES cells is warranted. The concept of ES cells arose through studies on mouse and human teratocarcinomas. These tumours were found to contain cells, called embryonal carcinoma cells, the stem [...]