In this article we will discuss about the applications of PCR. i. Detection of mutations: PCR is useful for detection of mutations related to genetic disease including point mutations, insertions and deletions. A notable example is that of the human dystrophin gene associated with Duchenne muscular dystrophy. The large-sized dystrophin gene consists of about two million base pairs located on [...]
The process of DNA cloning is carried out in four ways: 1. DNA Cloning Inside Host Cell 2. Chemically by Polymerase Chain Reaction (PCR) 3. Reproductive Cloning 4. Therapeutic Cloning. Way # 1. DNA Cloning Inside Host Cell: DNA cloning is the selective amplification of a specific DNA fragment or a sequence for producing large quantities of the DNA fragment [...]
In this article we will discuss about the meaning of transgenic mice. Cloned genes can be introduced into the germ line cells of an animal allowing study of the intact animal rather than in cultured cells. For this purpose, the nucleus in mouse embryonal cells has been used for injecting foreign DNA. The goal in such experiments is to have [...]