In this article we will discuss about the top two techniques used for introduction of foreign DNA without vectors. The techniques are: 1. Electroporation Technique 2. Microinjection Technique Some direct methods have also been developed for transferring DNA of one organism to a recipient without the participation of a vector. Two such methods are: 1. Electroporation technique and 2. Microinjection [...]
In this article we will discuss about the procedure for cloning recombinant DNA. The first step in a gene cloning programme is to construct a recombinant DNA molecule containing a donor DNA segment in which a selected gene is located and a vector DNA. The donor may belong to any taxonomic group. The DNA of the donor is cleaved into [...]
The following points highlight the top seven applications of recombinant DNA technology in molecular analysis of disease. The applications are: 1. Normal Gene Variations 2. Gene Variations Causing Disease 3. Point Mutations 4. Deletions, Insertions and Rearrangements of DNA 5. Pedigree Analysis 6. Restriction Fragment Length Polymorphism (RFLP) 7. Gene Therapy. Applications of Recombinant DNA Technology: Normal Gene Variations Gene [...]