In this article we will discuss about the organization of Ti Plasmid. Agrobacterium tumeifaciens harbours a large circular DNA known as Ti plasmid, which exists as an independent extra chromosomal entity of 200 kb size. The most spectacular regions of Ti plasmid are the presence of T (transfer) DNA and vir regions. Other components are origin of replication and regions [...]
The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound Mediated Transfer. Method # 1. Biolystic or Particle Bombardment: [...]
In this article we will discus about the strategies for stress-tolerant plant. Several microorganisms have been used as a model system to characterize stress inÂduced genes. It is because they are equipped with wide array of stress relieving genes. Recently, attention has been focused towards higher plants in search of stress tolerance genes and sucÂcessful attempt to introduce these genes [...]