Yeast episomal plasmids (YEps) are shuttle vectors. They can replicate in E. coli and also in yeast. The yeast episomal plasmids and has the following parts: 1. The 2 Micron (µm) Plasmid, 2. LEU2 Gene, and 3. The pBR 322. Part # 1. The 2 Micron (µm) Plasmid: This plasmid is found in several strains of yeast, Saccharomyces cerevisiae. It [...]
In this article we will discuss about the organization of Ti Plasmid. Agrobacterium tumeifaciens harbours a large circular DNA known as Ti plasmid, which exists as an independent extra chromosomal entity of 200 kb size. The most spectacular regions of Ti plasmid are the presence of T (transfer) DNA and vir regions. Other components are origin of replication and regions [...]
Everything you need to know about plasmids. Some of the frequently asked questions are as follows:- Q.1. What is a Plasmid ? Ans. The plasmids have been reported from prokaryotic as well as eukaryotic cells. A plasmid may be defined as extra-chromosomal genetic element which is not essential for the growth of the cell and has no extra cellular form. [...]