In this article we will discuss about the artificial synthesis of gene. For the first time in 1955, Michelson chemically synthesized a dinucleotide in laboratory. Later on in 1970, Har Govind Khorana and K.L. Agarwal for the first time chemically synthesized gene coding for tyrosine tRNA of yeast. For the synthesis of tRNA and rRNA there are specific genes. However, [...]
The below mentioned article provides notes on overlapping gene. In 1940s, Beadle and Tatum proposed one-gene-one protein hypothesis which explains that one gene encodes for one protein. However, if one gene consists of 1,500 base pairs, a protein of 500 amino acids in length would be synthesized. In addition, if the same sequence read in two different ways, two different [...]
In this article we will discuss about the discovery and evolution of split genes. Discovery of Split Genes: During 1970, in some mammalian viruses (e.g. adenoviruses) it was found that the DNA sequences coding for a polypeptide were not present continuously but were split into several pieces. Therefore, these genes were variously named as split genes or introns, interrupted genes [...]