In this article we will discuss about the concept and units of genes. Concept of Gene: Although the role of hereditary units (factors) in transfer of genetic characters over several generations in organisms was advocated by Gregor John Mendel, yet the mystery of the 'hereditary units' was un-ravelled during early 1900s. In 1909, W. Johanson coined the term 'gene' that [...]
In this article we will discuss about the concept, steps and applications of polymerase chain reaction (PCR). Concept of PCR: The polymerase chain reaction (PCR) provides a simple and ingenious method for exponentially amplification of specific DNA sequences by in vitro DNA synthesis. This technique was developed by Kary Mullis at Cetus Corporation between 1983 and 1985. This technique has [...]
In this article we will discuss about the artificial synthesis of gene. For the first time in 1955, Michelson chemically synthesized a dinucleotide in laboratory. Later on in 1970, Har Govind Khorana and K.L. Agarwal for the first time chemically synthesized gene coding for tyrosine tRNA of yeast. For the synthesis of tRNA and rRNA there are specific genes. However, [...]