The following points highlight the five main steps for aseptic manipulation. The steps are: 1. Sterilisation of the Culture Vessels and Instruments 2. Sterilisation of Nutrient Media 3. Sterilisation of Culture Rooms and Transfer-Area 4. Sterilisation of Explant 5. Aseptic Transfer of Explant/Sub-Culturing. Step # 1. Sterilisation of the Culture Vessels and Instruments: For sterilisation of culture vessels, glassware's, instruments, [...]
The following points highlight the top ten applications of plant cell and tissue culture. The applications are: 1. Clonal Propagation and Micro-Propagation 2. Biomass Energy 3. Secondary Metabolites 4. Genetic Variability 5. Somatic Embryogenesis and Synthetic Seed 6. Breaking Dormancy 7. Haploid Plants 8. Somatic Hybrids 9. Transgenic Plants 10. Germplasm Conservation. Application # 1. Clonal Propagation and Micro-Propagation: Plant [...]
The following four main steps of tissue culture techniques. The steps are: 1. Inoculation of Explant 2. Incubation of Culture 3. Sub-Culturing 4. Transplantation of the Regenerated Plant. Step # 1. Inoculation of Explant: Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the [...]