In this article we will discuss about the basic laboratory requirements for plant tissue culture. Laboratory Requirements: 'Plant tissue culture' or in vitro cultivation of plant parts needs some basic requirements: (a) Cultivation should be done under aseptic conditions. (b) The isolated plant part should get an appropriate environment which will help to divide the cell and to get an [...]
The below mentioned article provides a study note on totipotency. Cellular Totipotency: Though Haberlandt was able to culture isolated cells but failed to induce division in vitro. The culture of embryogenic tissue by Hannig (1904) and root-tip culture by Kotte and Robbins (1922) helped in solving the problems of cell divisions in in vitro conditions. During the period of 1934-1954, [...]
The below mentioned article provides a study note on culture media. Growth and morphogenesis of plant tissues in vitro are largely governed by the composition of the culture media. Although the basic requirements for culturing plant tissue is same but in practice there are some specific nutritional requirements for promoting optimal growth from different kinds of explants in case of [...]