In this article we will discuss about the basic laboratory requirements for plant tissue culture. Laboratory Requirements: 'Plant tissue culture' or in vitro cultivation of plant parts needs some basic requirements: (a) Cultivation should be done under aseptic conditions. (b) The isolated plant part should get an appropriate environment which will help to divide the cell and to get an [...]
The following points highlight the five main steps for aseptic manipulation. The steps are: 1. Sterilisation of the Culture Vessels and Instruments 2. Sterilisation of Nutrient Media 3. Sterilisation of Culture Rooms and Transfer-Area 4. Sterilisation of Explant 5. Aseptic Transfer of Explant/Sub-Culturing. Step # 1. Sterilisation of the Culture Vessels and Instruments: For sterilisation of culture vessels, glassware's, instruments, [...]
The below mentioned article provides a study note on totipotency. Cellular Totipotency: Though Haberlandt was able to culture isolated cells but failed to induce division in vitro. The culture of embryogenic tissue by Hannig (1904) and root-tip culture by Kotte and Robbins (1922) helped in solving the problems of cell divisions in in vitro conditions. During the period of 1934-1954, [...]