After reading this article you will learn about the meaning and system of plant tissue culture. Meaning of Tissue Culture: Tissue culture is a term, used to indicate the aseptic culture in vitro of a wide range of excised plant parts. It is used for both propagation and modification of genotypes (production of somaclonal variation and double haploids), biomass production [...]
In this article we will discuss about the basic laboratory requirements for plant tissue culture. Laboratory Requirements: 'Plant tissue culture' or in vitro cultivation of plant parts needs some basic requirements: (a) Cultivation should be done under aseptic conditions. (b) The isolated plant part should get an appropriate environment which will help to divide the cell and to get an [...]
The following four main steps of tissue culture techniques. The steps are: 1. Inoculation of Explant 2. Incubation of Culture 3. Sub-Culturing 4. Transplantation of the Regenerated Plant. Step # 1. Inoculation of Explant: Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the [...]