The following four main steps of tissue culture techniques. The steps are: 1. Inoculation of Explant 2. Incubation of Culture 3. Sub-Culturing 4. Transplantation of the Regenerated Plant. Step # 1. Inoculation of Explant: Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the [...]
The below mentioned article provides a study note on organogenesis. In plant tissue culture, organogenesis is a process of differentiation by which plant organs like roots, shoots, buds etc. are formed from the unusual points of origin of an organized explants where a preformed meristem is lacking. Plant development through organogenesis is the formation of organs either de novo or [...]
List of top two experiments on plant tissue culture:- 1. Isolation of Explants, Establishment and Maintenance of Callus 2. Culture of Anthers and the Establishment of Haploid Plants. Experiment # 1. Isolation of Explants, Establishment and Maintenance of Callus: Explants isolated from the tissues of higher plants are brought into culture. It requires a nutrient medium consisting of a mineral [...]