Archive | Gene

Confirmation of Transferred Gene | Genetics

However, even after transformation, it is essential to confirm the integration of genes, for the success of transgenesis. The incorporation can be confirmed through (i) The use of markers, (ii) Southern blotting, (iii) Northern blotting, and (iv) Polymerase chain reaction. (i) Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. It is, therefore, important that [...]

By |2016-11-02T05:49:18+00:00November 2, 2016|Gene|Comments Off on Confirmation of Transferred Gene | Genetics

Mutation: Definition, Detection and Types | Genetics

In this article we will discuss about:- 1. Definition of Mutation 2. History of Mutation 3. Aniridia in Man 4. Detection 5. Types. Contents: Definition of Mutation History of Mutation Aniridia in Man as a Case of Mutation Detection of Mutation Types of Mutation 1. Definition of Mutation: Heredity results accurate reproduction of genes. During cell-division the chromo­somes divide to [...]

By |2016-10-13T19:29:49+00:00October 13, 2016|Mutations|Comments Off on Mutation: Definition, Detection and Types | Genetics

Methods of Gene Transfer | Genetic Engineering

In this article we will discuss about the methods of gene transfer. Gene transfer can be done by two methods: (i) Indirect Method through Vectors or Carriers and (ii) Direct or Vectorless Transfer Method. The first method includes: (a) Plasmids and Viruses and (b) Natural Genetic Engineer, (a) Plasmids and Viruses: Recombinant DNA technology involves the transfer of desired genes [...]

By |2016-09-16T08:10:49+00:00September 16, 2016|Gene Transfer|Comments Off on Methods of Gene Transfer | Genetic Engineering
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