The below mentioned article provides a study note on gene sequencing. When a particular gene of interest or a DNA fragment is isolated, the sequencing of that piece of DNA becomes essential. The sequence of DNA refers to the order of nucleotide bases along its sugar phosphate backbone. No technique can determine the sequence of bases in an entire gene [...]
In this article we will discuss about the vectors of gene transfer. The common features of vectors used for transformation of eukaryotic cells include: (i) Multiple cloning sites (unique restriction site), (ii) Bacterial origin of replication, and (iii) Selectable marker gene which will allow to select the proper transformed cell. Ti and Ri Plasmid: The potential use of the Agrobacterium [...]
The following points highlight the top two methods of gene transfer. The methods are: 1. Indirect Method (Agrobacterium Mediated Gene Transfer) 2. Direct Methods of Gene Transfer. Method # 1. Indirect Method (Agrobacterium Mediated Gene Transfer): The transformation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in [...]