The following points highlight the top six uses of mutations in microorganisms. The uses are: 1. Determination of Function 2. Demonstration of Metabolic Pathways in Microorganisms 3. For Understanding The Metabolic Regulation 4. For Matching a Biochemical Entity with a Biological Function 5. For Locating the Site of Action of External Agents 6. For the Production of Useful Products. Use [...]
The following points highlight the four methods to detect and isolate mutants. The methods are: 1. Replica Plating Technique 2. Resistance Selection Method 3. Substrate Utilization Method 4. Carcinogenicity Test. 1. Replica Plating Technique: Lederberg and Lederberg (1952) have given replica plating technique. This technique is used to detect auxotrophic mutants which differentiates between mutants and wild type strains on [...]
In this article we will discuss about the regulation of gene expression in prokaryotes and eukaryotes. The DNA of a microbial cell consists of genes, a few to thousands, which do not express at the same time. At a particular time only a few genes express and synthesize the desired protein. The other genes remain silent at this moment and [...]