In this article we will discuss about the regulation of gene expression in prokaryotes and eukaryotes. The DNA of a microbial cell consists of genes, a few to thousands, which do not express at the same time. At a particular time only a few genes express and synthesize the desired protein. The other genes remain silent at this moment and [...]
Some procedures applied for gene cloning in eukaryotes are described in this context: 1. Transformation of Filamentous Fungi 2. Gene Transfer through Ti-plasmid of Agrobacterium 3. Gene Transfer through Gene Gun 4. Gene Bank or Genomic Library. 1. Transformation of Filamentous Fungi: Like bacteria, transformation of filamentous fungi has also been demonstrated. Hinnen (1978) first described transformation of protoplasts of [...]
The following points highlight the top four applications of gene technology. The applications are: 1. Production of Pharmaceuticals 2. Diagnosis of Diseases 3. Insect Pest Control 4. Use of Genetically Engineered Microorganisms for Control of Pollution. Application # 1. Production of Pharmaceuticals: The production of medically useful human peptides and proteins (e.g. human growth hormones, insulin, somatostatin and interferon) are [...]