In this article we will discuss about the DNA extraction and amplification. Normally, CTAB (cetyl trimethyl ammonium bromide) method is used. However, SDS method is also common and is described as follows: 1. The leaves are washed with sterilised distilled water and blot dried and cut into small pieces with a sterilised blade. 2. 1g of leaf material is placed [...]
After reading this article we will learn about the consequences and co-efficient of inbreeding. The mating of individuals more closely related than individuals mating at random is known as inbreeding. The most intense form of inbreeding is selfing. Consequences of Inbreeding: (i) Reduction in genetic variance within a small population (ii) General decrease in over-all heterozygosity (iii) Differentiation of a [...]
Most molecular markers fall into one of three basic categories of techniques that use either hybridization or are based on the polymerase chain reaction (PCR): Category # 1: Hybridization Based (non-PCR) Techniques: Category I includes restriction fragment length polymorphism (RFLP) analysis, where probes are hybridized to filters containing DNA which has been digested with restriction enzymes. The resultant fragments are [...]