Most molecular markers fall into one of three basic categories of techniques that use either hybridization or are based on the polymerase chain reaction (PCR): Category # 1: Hybridization Based (non-PCR) Techniques: Category I includes restriction fragment length polymorphism (RFLP) analysis, where probes are hybridized to filters containing DNA which has been digested with restriction enzymes. The resultant fragments are [...]
After reading this article you will learn about the concept and methods of stability. Concept of Stability: The term 'phenotypic stability', yield stability, and adaptation are often used in quite different senses. Therefore, Dorst (1957) remarked that "the word adaptation has great adaptability" and Lin (1986) amplified that "it (the concept of stability) is defined in many ways depending on, [...]
In this article we will discuss about agarose gel electrophoresis. To know about quality and quantity of genomic DNA, agarose gel electrophoresis is performed on genomic and amplified DNA. Larger molecules migrate more slowly because of greater friction drag and they form their way through the pores of gel, less efficiently than smaller molecules. Range of separation in gel containing [...]