The below mentioned article provides a study note on totipotency. Cellular Totipotency: Though Haberlandt was able to culture isolated cells but failed to induce division in vitro. The culture of embryogenic tissue by Hannig (1904) and root-tip culture by Kotte and Robbins (1922) helped in solving the problems of cell divisions in in vitro conditions. During the period of 1934-1954, [...]
The following four main steps of tissue culture techniques. The steps are: 1. Inoculation of Explant 2. Incubation of Culture 3. Sub-Culturing 4. Transplantation of the Regenerated Plant. Step # 1. Inoculation of Explant: Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the [...]
The following points highlight the top eight types of tissue culture. The types are: 1. Seed Culture 2. Embryo Culture 3. Meristem Culture 4. Bud Culture 5. Callus Culture 6. Cell Suspension Culture 7. Anther Culture 8. Protoplast Culture. Type # 1. Seed Culture: Seeds may be cultured in vitro to generate seedlings or plants. It is the best method [...]