In this article we will discuss about:- 1. Selection of Disease Resistant Variants at the Plant Level 2. Selection in Vitro with Toxins and Culture Filtrates against Diseases 3. Selection for Resistance to Drought and Flooding 4. Selection for Salt Tolerance. Selection of Disease Resistant Variants at the Plant Level: One approach to utilise somaclonal variation is to screen regenerated [...]
The below mentioned article provides a study note on culture media. Growth and morphogenesis of plant tissues in vitro are largely governed by the composition of the culture media. Although the basic requirements for culturing plant tissue is same but in practice there are some specific nutritional requirements for promoting optimal growth from different kinds of explants in case of [...]
The following points highlight the five main steps for aseptic manipulation. The steps are: 1. Sterilisation of the Culture Vessels and Instruments 2. Sterilisation of Nutrient Media 3. Sterilisation of Culture Rooms and Transfer-Area 4. Sterilisation of Explant 5. Aseptic Transfer of Explant/Sub-Culturing. Step # 1. Sterilisation of the Culture Vessels and Instruments: For sterilisation of culture vessels, glassware's, instruments, [...]