Let us make an in-depth study of the polymerase chain reaction, which is a technique for amplifying DNA sequences. The below given article will help you to understand the following things:- 1. Polymerase Chain Reaction Amplifies Specific Regions of DNA 2. Performing a Polymerase Chain Reaction 3. Taq Polymerase Simplifies and Improves the Polymerase Chain Reaction and Others. The polymerase [...]
In this article we will discuss about the concept, steps and applications of polymerase chain reaction (PCR). Concept of PCR: The polymerase chain reaction (PCR) provides a simple and ingenious method for exponentially amplification of specific DNA sequences by in vitro DNA synthesis. This technique was developed by Kary Mullis at Cetus Corporation between 1983 and 1985. This technique has [...]
In this article we will discuss about the Polymerase Chain Reaction:- 1. Steps of Polymerase Chain Reaction 2. Advantages of Polymerase Chain Reaction 3. Applications. Steps of Polymerase Chain Reaction: PCR uses DNA polymerase to amplify repetitively targeted portions of DNA. The amplified DNA sequence can then be analysed by southern hybridization. i. Primer Construction: (a) It is essential to [...]