In this article we will discuss about:- 1. Gene Expression in Vegetative Organs 2. Gene Expression in Reproductive Organs 3. Gene Expression in Seeds 4. Gene Expression in Fruits. Successful studies on gene expression in plants are undoubtedly due to the contribution of early botanical monograph in which cytological structure and function of organs were defined. With the advent of [...]
The following points highlight the top six plant promoters for genes. The promoters are: 1. Core Promoter 2. Light Responsive Promoters 3. Stress Responsive Promoters 4. Plant Hormonal Responsive Promoters 5. Sucrose Responsive Promoters 6. Low-Oxygen Responsive Promoters. 1. Core Promoter: Core promoter elements are defined as minimal promoter that contain TATA box. These are indispensable for initiation of transcription [...]
Generally, isolation of protoplast is categorized into mechanical and enzymatic. Also learn about the techniques involved in the isolation of leaf mesophyll protoplast. Mechanical Method: This method is crude and oldest method for protoplast isolation. It is only an historical perspective. It involves chopping and plasmolysis of tissues. Time consuming and low yield of protoplast are the main constraints in [...]