In this article we will discuss about:- 1. Gene Expression in Vegetative Organs 2. Gene Expression in Reproductive Organs 3. Gene Expression in Seeds 4. Gene Expression in Fruits. Successful studies on gene expression in plants are undoubtedly due to the contribution of early botanical monograph in which cytological structure and function of organs were defined. With the advent of [...]
The following points highlight the top six plant promoters for genes. The promoters are: 1. Core Promoter 2. Light Responsive Promoters 3. Stress Responsive Promoters 4. Plant Hormonal Responsive Promoters 5. Sucrose Responsive Promoters 6. Low-Oxygen Responsive Promoters. 1. Core Promoter: Core promoter elements are defined as minimal promoter that contain TATA box. These are indispensable for initiation of transcription [...]
The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]