In this article we will discuss about: 1. Process of Somatic Embryogenesis 2. Embryo Maturation and Synchronisation 3. Cultural Conditions 4. Recurrent Embryogenesis and Mass Production 5. Applications. After fertilization, zygote is transformed into adult status through a series of embryogenic processes. Despite the same genetic constituents, somatic cells on the other hand, do not reorient towards embryo production. However, [...]
The following points highlight the three main factors affecting micro-propagation. The factors are: 1. Genotype 2. Media 3. Cultural Conditions. Factor # 1. Genotype: Physiological conditions of the donor plants influence considerably in the process of morphogenesis. Larger sizes of the meristematic tissues are advantageous. Selecting juvenile parts of the plants yield better results than older ones. The most actively [...]
Generally, isolation of protoplast is categorized into mechanical and enzymatic. Also learn about the techniques involved in the isolation of leaf mesophyll protoplast. Mechanical Method: This method is crude and oldest method for protoplast isolation. It is only an historical perspective. It involves chopping and plasmolysis of tissues. Time consuming and low yield of protoplast are the main constraints in [...]