Generally, isolation of protoplast is categorized into mechanical and enzymatic. Also learn about the techniques involved in the isolation of leaf mesophyll protoplast. Mechanical Method: This method is crude and oldest method for protoplast isolation. It is only an historical perspective. It involves chopping and plasmolysis of tissues. Time consuming and low yield of protoplast are the main constraints in [...]
Fusion of protoplasts can be accomplished by two distinct methods like chemical fusion and electro-fusion (Fig. 10.1). Earliest method known as mechanical fusion, where protoplasts are forced to fuse under applied pressure within the pipette. Protoplast in suspension can undergo spontaneous fusion in cultural conditions. Method # 1. Chemical Fusion of Protoplast: Chemicals such as sodium nitrate, polyethylene glycol and [...]
In this article we will discuss about the significance of haploids. Application in Crop Improvement: Productions of homozygous pure lines are the major goal in breeding strategy. These are obtained by back crossing and inbreeding. These processes are however, are time consuming. Once established, homozygous lines have potential for plant improvement in several ways. With the advent of anther culture, [...]