In this article we will discuss about:- 1. Subject-Matter of Restriction Enzymes 2. Nomenclature of Restriction Enzymes 3. Types 4. Sites 5. Restriction Enzymes Generated Staggered and Blunt Ends 6. Purification. Subject-Matter of Restriction Enzymes: Restriction enzymes, also known as restriction endonucleases, have played a key role in the development of recombinant DNA technology. These have been found in microorganisms [...]
The sequence of a deoxyribonucleic acid DNA molecule can be elucidated using chemical or enzymatic methods. Chemical Method of DNA Sequencing: In 1997, Maxam and Gilbert of Harward University discovered this method. In this method, a DNA fragment to be sequenced is radiolabeled at one end of molecule (Fig. 13.15). After denaturation of DNA into single strand, bases are modified [...]
Although substantial progress was made during the last decade, all replication mechanisms in eucaryotes are not yet understood because: (a) Replication is an event which occurs much more rarely than in bacteria. Initiation of replication is therefore highly controlled; this complicates the understanding of this process; (b) The DNA is not naked, in the cell, but in the form of [...]