In this article we will discuss about the role of RNA in transcription regulation. In the early 1960s, Francois Jocob and Jaques Monod performed exemplary work pertaining to prokaryotic gene regulation. These experimental techniques and strategies were later extended to eukaryotic system. Although control of transcription between prokaryotic and eukaryotic genes shares substantial homology, eukaryotic control of system is highly [...]
In this article we will discuss about:- 1. Subject-Matter of Restriction Enzymes 2. Nomenclature of Restriction Enzymes 3. Types 4. Sites 5. Restriction Enzymes Generated Staggered and Blunt Ends 6. Purification. Subject-Matter of Restriction Enzymes: Restriction enzymes, also known as restriction endonucleases, have played a key role in the development of recombinant DNA technology. These have been found in microorganisms [...]
Although substantial progress was made during the last decade, all replication mechanisms in eucaryotes are not yet understood because: (a) Replication is an event which occurs much more rarely than in bacteria. Initiation of replication is therefore highly controlled; this complicates the understanding of this process; (b) The DNA is not naked, in the cell, but in the form of [...]