In this article we will discuss about SIRPH - A Rapid, Quantitative SNuPE-IP RP HPLC Method for Simultaneous Methylation Analysis at Multiple CpG Sites. Introduction: Bisulfite Based DNA Methylation Analysis: The most widely used protocols for detailed DNA methylation analyses are based on the use of bisulfite converted genomic DNA. This methodology was introduced by M. Frommer in the early [...]
The following points highlight the two types of gel electrophoresis. The types are: 1. Polyacrylamide Gel Electrophoresis 2. Agarose Gel Electrophoresis Gel electrophoresis is the novel technique in which nucleic acid (even proteins) molecules are separated based on the size differences when subjected to electric field. In electrophoresis, the rate of migration in electric field depends on the field, net [...]
In this article we will discuss about the role of RNA in transcription regulation. In the early 1960s, Francois Jocob and Jaques Monod performed exemplary work pertaining to prokaryotic gene regulation. These experimental techniques and strategies were later extended to eukaryotic system. Although control of transcription between prokaryotic and eukaryotic genes shares substantial homology, eukaryotic control of system is highly [...]